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Investigation of parasitic fleas on wild rodents in Zhangjiakou competition zone of the 24th Olympic Winter Games, 2018-2021
CHEN Yong-ming, KANG Dong-mei, LAN Xiao-yu, YAN Dong, LIU Guan-chun, SHI Xian-ming, DU Guo-yi, ZHOU Song, YANG Shun-lin, HOU Zhi-lin, ZHENG Nan, YANG Ai, ZHANG Xiao-lei, ZHANG Jin-yi, SUN Fei, MA Yong, GUO Chao, DAI Hong, WANG Xiao-yan
Abstract137)      PDF (822KB)(578)      
Objective To investigate the changes in the number and composition of parasitic flea populations on wild rodents in the Zhangjiakou competition zone of the 24th Olympic Winter Games from 2018 to 2021, and to provide a basis for prediction and warning of rodent-borne diseases in the in Chongli district, Zhangjiakou competition zone. Methods Wild rodents were caught by the night traping method in Chongli district, Zhangjiakou city. Parasitic fleas on the rodents were collected and identified. Excel 2010 software was used to summarize the data and perform year-by-year and month-by-month analyses. A chordal graph was used to present the relationship between wild rodents and parasitic fleas. The seasonal distribution of fleas was analyzed by the concentration degree method. Results From 2018 to 2021, a total of 302 rodents were caught in Chongli district, belonging to 11 species of 9 genera under 4 families. All rodent species but Mus musculus carried fleas. A total of 218 fleas were collected, belonging to 13 species of 8 genera under 4 families. The average flea index was 0.72. The most dominant flea species was Amphipsylla vinogradovi, which had the closest relationship with Cricetulus barabensis and was also found on other seven rodent species including C. longicaudatus, Tscherskia triton, and Apodemus agrarius. Strong seasonality (all M=0.72) was observed in total, male, and female parasitic fleas on wild rodents. Conclusion Parasitic fleas on wild rodents in Zhangjiakou competition zone have large quantities, diverse species, and strong seasonality, with a close relationship with C. barabensis. Their population density and structure changes should be a warning in key areas. Attention should be paid to the trend and complications of rodent-borne disease epidemics.
2022, 33 (3): 414-417.   doi: 10.11853/j.issn.1003.8280.2022.03.019
Investigation of small mammals from 2018 to 2021 in the Zhangjiakou competition zone of the 24th Olympic Winter Games
CHEN Yong-ming, KANG Dong-mei, DU Guo-yi, YAN Dong, LIU Guan-chun, ZHOU Song, ZHENG Nan, LAN Xiao-yu, YANG Ai, MA Yong, ZHANG Xiao-lei, WEN Lin, SUN Fei, WANG Xiao-yan
Abstract140)      PDF (695KB)(576)      
Objective To understand the changes in the size and structure of small mammals populations from 2018 to 2021 in the Zhangjiakou competition zone of the 24th Winter Olympic Games, and to provide the basis for the prediction and warning of small mammals-related diseases in this area. Methods Field investigation of small mammals was carried out in two towns and eight townships of Chongli district, which are the locations of facilities of the Zhangjiakou competition zone of the 24th Winter Olympic Games. Using the 5 m clamp line method, 6-8 sampling sites were selected randomly every month, and 1-2 representative habitats with different type or altitude were selected at each site (100 mousetraps per habitat). Excel 2010 software was used to summarize the monthly and yearly survey data of small mammals. By using the methods of concentration degree and linear correlation, the seasonal distribution of small mammals and the distribution characteristics in different habitats were analyzed. Results In 2018-2021, 438 small mammals (hereinafter referred to as rodents) were captured using 33 610 clamp-times, with an average capture rate of 1.30%. The rodents belonged to 2 orders, 4 families, 9 genera, and 12 species, with Apodemus agrarius as the dominant species (125, 28.54%). The year with the highest rodent density was 2019, and the capture rate was 2.09%. The year with the highest number of rodent species was 2020, and 12 species were captured. The density of rodents showed large seasonal variations ( M=0.58) in a bimodal pattern, with the first peak in March of spring and the second peak in August of autumn. The species of rodent showed seasonal variations ( M=0.31) in a unimodal pattern. The density of rodents increased with elevation, and the capture rate was 0.22% (low elevation), 1.06% (medium elevation), and 2.00% (moderately high elevation). Most species of rodents were captured at medium elevation (10 species), followed by moderately high elevation (4 species) and low elevation (3 species). There was a positive correlation between rodent density and species in different habitats ( r=0.823, P=0.006). The changes of rodent density and species were most significant in grassland, farmland, forest belt, and hillside. Conclusion In the Chongli district in Zhangjiakou competition zone of the 24th Winter Olympic Games, rodents have high population, rich species composition, and strong seasonal variation. The distribution of rodents in different habitats showed specific patterns. The changes in rodent density and species composition in key areas should be monitored. Attention should be paid to epidemic trend of rodent-borne diseases and the important influence of their transmission.
2022, 33 (2): 293-296.   doi: 10.11853/j.issn.1003.8280.2022.02.024
An investigation of the species composition and distribution of rodents and their parasitic fleas in the Winter Olympic Games zone and surrounding areas in Zhangjiakou, Hebei province, China
YAN Dong, CHEN Yong-ming, SUN Fei, MA Yong, ZHOU Song, LIU Guan-chun, YANG Shun-lin, GUO Chao, DAI Hong, ZHANG Jin-yi, ZHANG Xiao-lei, WEN Lin, WANG Xiao-yan, DU Guo-yi, SHI Xian-ming
Abstract315)      PDF (551KB)(996)      
Objective To investigate the species composition, quantitative distribution, and seasonal variation of rodents and their parasitic fleas in the Zhangjiakou 2022 Winter Olympic Games zone (hereinafter referred to as the Zhangjiakou competition zone) and the surrounding areas in Hebei province, China, and to provide basic data for the prediction and emergency treatment of vector-borne diseases during the Winter Olympic Games. Methods From September 2018 to September 2019, the five-meter traping method and the quadrat method were used to investigate the species composition and density of rodents in Chongli district and the area around the Zhangjiakou competition zone, and parasitic fleas were collected and classified. The ecological method was used to analyze the species composition, distribution, and seasonal density variation of rodents, and Excel 2010 software was used to analyze data and draw charts. Results The quadrat method was used to investigate 20 quadrats, and no rodents were captured. The five-meter traping method was used to investigate 166 sites, and 266 rodents were captured by 16 600 traps and were classified into 8 species, 5 genera, and 3 families, with a capture rate of 1.60%. Time variation showed a rodent density of <1.00% from October to June in the next year, especially from December to April in the next year. Apodemus agrarius and Cricetulus barabensis were the main rodent species, and the rodent density was 6.14% in July and 5.93% in September. There was a variety of rodent species, mainly A. agrarius, C. migratorius, C. barabensis, A. peninsulae, and A. draco. A total of 266 rodents were examined, among which 36 were found to have fleas, with a flea infestation rate of 13.53%, and a total of 100 fleas were collected and classified into 7 species, 5 genera, and 3 families, with a mean flea index of 0.38. Bacteriological and serological tests were performed for all materials and yielded negative results. Conclusion Main host animals and fleas in Hebei province and surrounding natural plague foci are not found in the Zhangjiakou competition area and the surrounding area, and there are differences in natural landscape, the species composition of rodents, and the composition of fleas between the Zhangjiakou competition zone and natural plague foci in Hebei province. Therefore, there is a relatively low risk of primary animal plague in the local area.
2021, 32 (2): 161-164.    doi: 10.11853/j.issn.1003.8280.2021.02.008
Attracting effect of fecal extract of Blattella germanica on themselves
WANG Xiao-Yan, YOU Hong, ZHU Ao, ZHU Fu-Xing
Abstract1242)      PDF (347KB)(1015)      

Objective Attracting effect of fecal extract of the Blattella germanica of different ages and genders on themselves was measured in the present study in order to provide more effective guidance on the chemical control of B. germanica. Methods B. germanica feces were immersed in organic solvents to produce the extract, of which the attracting effect was measured by a four?arm olfactometer. Results The ethanol fecal extract of early nymphs was the best to attract early nymphs, late nymphs, male adults and female adults, with the attraction rates of 39.72%, 40.56%, 38.33% and 34.17%, respectively, followed by fecal extract of late nymphs, the attraction rates of which were 30.56%, 30.56%, 27.22% and 26.11%, respectively. The extracts of male and female adult feces were the worst at attracting, though the one of male adult feces had an attraction rate of 22.22% to female adults, and that of the female adult fecal extract reached 24.72% to male adults, both higher than the effect of nymph fecal extracts and that between the same gender. The effectiveness of toxic baits increased significantly when added a certain amount of the ethanol extracts of early or late nymph feces; the 24 h kill rate of deltamethrin baits increased from 26.00% to 54.00% and 48.00%, respectively; the 24 h kill rate of phoxim baits increased from 20.00% to 48.00% and 40.00%, respectively. Conclusion The fecal extract of early nymphs had a favorable attracting effect on the nymphs of all ages and male and female adults, while the fecal extract of a certain gender satisfyingly attracted the opposite. Mixed with the ethanol fecal extract of early and late nymph, the toxic baits demonstrated improvement in the killing efficiency.

2010, 21 (3): 201-203.
Attractiveness of different foods for Blattella germanica
WANG Xiao-Yan, ZHU Ao, YOU Hong, ZHU Fu-Xing
Abstract1273)      PDF (389KB)(1007)      

Objective To determine the attractiveness of different foods and identify an effective attractant that can act as a toxic bait for chemical control for Blattella germanica. Methods The four?arm olfactometer was used to sort out strongly attractive foods from a variety of edible materials for B. germanica. The candidate was then prepared with insecticides into toxic baits. Results The strongest attractants identified from four groups were fried soybean powder, cabbage juice, bananas and glucose, with attracting rates of 63.33%, 59.44%, 63.33% and 42.78%, respectively. The four best attractive ingredients were subject to the optimal luring test, which showed that fried soybean powder had the greatest attracting effect on B. germanica with the attracting rate of 45.66%, followed by bananas (25.83%). Combined with deltamethrin or Phoxim, bananas could act as the most efficiently attractive toxic bait. Conclusion The screening study showed that soybean powder and bananas had strong attractiveness for B. germanica. Bananas can be used to produce the most effective toxic bait.

2010, 21 (2): 112-114.
Research on activities of carboxylesterase,glutathione  S?transferase  and  acetylcholinesterase  in  different development stages and gender adults of Blattella germanica
ZHU Ao, YiU Hong, WANG Xiao-Yan, ZHU Fu-Xing
Abstract1926)      PDF (429KB)(1538)      

  【Abstract】 Objective To study activity variance of carboxylesterase,glutathione S?transferase and acetylcholinesterase in different development stages and gender adults of German cockroach (Blattella germanica). Methods Enzyme activities were measured by spectrophotometry in vitro and the data were analyzed with software DPS. Results The activities of carboxylesterase and acetylcholinesterase in the first?instar nymphae were the highest and the activities values were (0.3741±0.0077)μmol/(mg pro·min) and  (61.7586±9.2400)nmol/(mg pro·min), respectively. The differences among different development instars were significant. The activity of carboxylesterase in male adults was higher than that in female adults, and the difference between them was significant. For acetylcholinesterase, the activity in male adults was higher than that in female adults, but the difference was not significant.  The  activity  of  glutathione  S?transferase  among  different  development  stages  and  gender  adults  had  almost  no difference. Conclusion The activity differences of carboxylesterase and acetylcholinesterase among different development stages and male, female adults were significant whereas that  of glutathione S?transferase was not significant.

2009, 20 (5): 423-426.
Susceptibility of different instars and sexes of Blattella germanica to pyrethroid insecticides
ZHU Ao, YOU Hong, WANG Xiao-Yan, ZHU Fu-Xing
Abstract1227)      PDF (291KB)(1121)      

【Abstract】 Objective To assay the toxicities of deltermethrin and cypermethrin to male, female adults and the first, third, fifth instar nymphae of Blattella germanica, and to provide the effective instruction for chemical control of B.germanica. Methods The  insecticide  films  in  jar  was  used  in this study. Results There was significant difference for the tolerance of B.germanica to deltamethrin and cypermethrin  among different instars and different sexes. The tolerance of the first instar nymphae to deltamethrin and cypermethrin was the lowest with the KT50 values of 7.1743 min and 10.0015 min respectively. The KT50 of the fifth instar nymphae to these two chemicals were 14.1624 min and 24.6041 min respectively, highest in all the instars. The tolerance level of nymphae increased gradually with the development of instars. The tolerance level of male adults was higher than that of female adults. The KT50 of male adults to the two tested chemicals were 9.6157 min and 11.7492 min respectively. Conclusion The KT50 of the fifth instar nymphae to deltermethrin and cypermethrin was the highest. The KT50 of male adults to deltermethrin and cypermethrin was higher than that of female adults.

2009, 20 (2): 127-128.